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In many gene amplification applications, it is important to detect point mutations present in a sample. If a mutation is present in a specific gene, the genotype of the individual may be indicative of a disease state. In most cases, it is desirable that the method used to detect the mutation is simple, specific, and requires little or no special equipment. Polymerase chain reaction (PCR) is a commonly used method of detecting mutations in nucleic acids. It relies on the ability of the PCR enzyme to replicate DNA to create large numbers of copies. However, even with this amplification step, mutations that differ in sequence from the wild type can be difficult to detect because of the presence of homologous sequences in the wild type nucleic acid. Because of this homology, it is desirable that the method used to detect mutations rely on information that is different than the information used to generate the mutation. Here, we demonstrate that the use of palindromic oligonucleotides in the PCR amplification step can be used to detect specific point mutations in a PCR amplicon.--TEST--
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